human prostate tissue slides  (BioChain Institute)

Journal: International Journal of Molecular Sciences

Article Title: Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression

doi: 10.3390/ijms23084247

Figure Lengend Snippet: Increased infiltrating macrophages in the prostate intraepithelial neoplasia were M2 subtype. ( A ) Human tissue samples of prostate cancer containing the area of prostate intraepithelial neoplasia (PIN) were immunostained with the antibody of CD68, a pan marker for macrophages (top panel, and middle panel: zoom-in areas). Hematoxylin and eosin (H & E) staining was carried out to visualize the morphology of the prostate cancer tissues (bottom panel). Scale bar: 25 µm. ( B , C ) Raw 264.7 macrophages cultured with either control media or Pr111 PIN complete media were fixed, permeabilized, and immunostained with antibodies for markers of pan macrophages, F4/80; M1 macrophages including iNOS ( B ) and CD38 ( C ); M2 macrophages including Ym1 ( B ) and CD206 ( C ). Scale bar: 20 µm. ( D ) Human tissue samples of PIN were immunostained with antibodies of CD68 and CD206 for the assessment of all macrophages (green) and M2 macrophages (red), respectively. Nuclei were labeled by DAPI and visualized. Scale bar: 50 µm. The image shown here is representative of at least 3 independent experiments or tissue samples.

Article Snippet: Human prostate tissue slides, including normal or cancer tissue samples, were obtained from BioChain (Newark, CA, USA), US Biomax (Derwood, MD, USA), and Cooperative Human Tissue Network (CHTN).

Techniques: Marker, Staining, Cell Culture, Control, Labeling

Journal: International Journal of Molecular Sciences

Article Title: Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression

doi: 10.3390/ijms23084247

Figure Lengend Snippet: Expression of Spp1 receptors in PIN cells. ( A ) Cell lysates from murine PIN Pr111 cells grown on Matrigel in 3D treated with recombinant Spp1 or control for 72 h were subjected to immunoblotting for assessment of the Spp1 receptors, including integrin αvβ3, integrin β1, and CD44. Long exposure: longer time to catch the emitted signal from the WB membrane on the X-ray film. ( B – E ) Human tissue samples of normal prostate and prostate cancer containing PIN areas were immunostained for the expression of CD44 ( B ), integrin αv ( C ), integrin β1 ( D ), and integrin β3 ( E ). Scale bar: 50 µm. the image shown here is representative of at least 3 independent experiments or tissue samples.

Article Snippet: Human prostate tissue slides, including normal or cancer tissue samples, were obtained from BioChain (Newark, CA, USA), US Biomax (Derwood, MD, USA), and Cooperative Human Tissue Network (CHTN).

Techniques: Expressing, Recombinant, Control, Western Blot, Membrane

Journal: International Journal of Molecular Sciences

Article Title: Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression

doi: 10.3390/ijms23084247

Figure Lengend Snippet: Macrophage Spp1 activated Akt and JNK signaling in PIN cells. ( A – C ) Pr111 cells cultured on Matrigel in 3D were stimulated with either control/ddH 2 O or recombinant Spp1 for 72 h. Cell lysates were collected and subjected to immunoblotting to examine the proteins of interest, including p-Akt, Akt, p-ERK, and ERK ( A ); p-JNK, JNK, p38 MAPK, p38 MAPK, p-Src, and Src ( B ); and IκBα ( C ). The relative intensity fold change was calculated by the ratio of phosphorylated protein levels over the corresponding total protein levels and set as 1 for the control in each case. Each image shown is representative of 3–5 independent experiments. ( D ) Human tissue samples of normal prostate and prostate cancer containing PIN areas were immunostained for phospho-Akt expression. Scale bar: 25 µm. n = 3.

Article Snippet: Human prostate tissue slides, including normal or cancer tissue samples, were obtained from BioChain (Newark, CA, USA), US Biomax (Derwood, MD, USA), and Cooperative Human Tissue Network (CHTN).

Techniques: Cell Culture, Control, Recombinant, Western Blot, Expressing

Journal: International Journal of Cancer

Article Title: Olfactomedin 4 downregulation is associated with tumor initiation, growth and progression in human prostate cancer

doi: 10.1002/ijc.32535

Figure Lengend Snippet: Reduced OLFM4 expression is associated with higher Gleason scores and lower recurrence‐free survival in human primary prostate adenocarcinoma. ( a ) Representative images of HE staining and immunohistochemistry (IHC) analysis of OLFM4 protein expression in adjacent normal and tumor regions of whole‐mount section human prostate cancer tissue specimens (obtained from the Laboratory of Pathology, National Cancer Institute). All micrographs shown are for tissues obtained from the same case. LT, lower grade tumor (Gleason grade 3, GL. 3); HT, higher grade tumor Gleason grade 4, GL. 4). Scale bars: 50 μm. ( b ) Quantitation of OLFM4 protein expression from immunohistochemical analyses using human prostate cancer tissue slides obtained from CHTN and US Biomax. Data represent the mean ± standard deviation (SD) of 3,3′‐diaminobenzidine (DAB) intensity normalized to the number of nuclei. Adjacent Nor., normal tissue adjacent to primary tumor; Gleason score ≤4 + 3; Gleason score ≥4 + 4. We excluded CHTN and US Biomax cases with quality issues for which we could not obtain immunohistochemistry data. *** p ≤ 0.001 (ANOVA). ( c ) OLFM4 mRNA expression in prostate cancer specimens in data downloaded from the GSE21032 dataset. Data represent the mean ± SD. Normal, normal tissue adjacent to primary tumor; P‐tumor, primary tumor; M‐PCs; prostate tumor with distant metastasis. ** p ≤ 0.01; *** p ≤ 0.001 (ANOVA). ( d ) Kaplan–Meier plot of recurrence‐free survival for OLFM4 mRNA higher‐expressing (red line) and lower‐expressing (blue line) prostate adenocarcinoma patient cohorts in the GSE21032 dataset at 25% thresholds ( p = 0.0000154; log‐rank test).

Article Snippet: Human prostate cancer tissue array slides (for 70 prostate cancer cases and 10 normal tissues) were purchased from US Biomax (PR803, Rockville, MD).

Techniques: Expressing, Staining, Immunohistochemistry, Quantitation Assay, Immunohistochemical staining, Standard Deviation